Robert-Bunsen-Vorlesung 2020


Robert-Bunsen-Vorlesung 2020


Prof. Dr. Roland Winter, Technische Universität Dortmund

„Interrogating the Free-Energy Landscape and Structural Dynamics of Biomolecular Systems by Pressure Perturbation

findet statt am

Mittwoch, den 21. Oktober 2020 um 17.15 Uhr
an der Philipps-Universität Marburg FB 15 Chemie, Hörsaal C

Hans-Meerwein-Straße 4

35043 Marburg

Roland Winter (*1954) ist seit 1993 Professor an der Fakultät für Chemie und Chemische Biologie (Bereich Physikalische Chemie) der TU Dortmund. Er ist Mitglied des Executive Boards des Exzellenzclusters RESOLV (Ruhr Explores Solvation) und leitet die DFG-Forschungsgruppe "Exploring the Dynamical Landscape of Biomolecular Systems by Pressure Perturbation". Weitere Informationen über Professor Winter und seine Forschungsaktivitäten finden Sie unter


Abstract: High hydrostatic pressure affects the structure, dynamics, and stability of biomolecular systems and is a key parameter in the context of the exploration of the origin and the physical limits of life [1]. Pressure perturbation also serves as an important tool to mechanistically explore the conformational and free-energy landscape of biomolecular systems [2]. By combining X-ray and neutron scattering, calorimetry, spectroscopies, microscopy and theoretical approaches, the combined effects of temperature, pressure and cosolutes on the structure, solvational properties, dynamics and intermolecular interactions of proteins and other biomolecules will be discussed [3-7]. Pressure-jump relaxation studies are used to study the kinetics and mechanisms of biomolecular phase transitions and structural transformations, such as membrane fusion or protein and nucleic acid folding. Finally, the advantages of using pressure to explore biomolecular assemblies, liquid-liquid phase separation phenomena of proteins and to modulate enzymatic reactions are discussed [8,9]

[1] I. Daniel, P. Oger, R. Winter, Origins of Life and Biochemistry under High-pressure Conditions. Chem. Soc. Rev. 35 (2006) 858-875

[2] R. Winter, Interrogating the Structural Dynamics and Energetics of Biomolecular Systems with Pressure Modulation, Ann. Rev. Biophys. 48 (2019) 441-461

[3] S. Suladze, S. Cinar, B. Sperlich, R. Winter, Pressure Modulation of the Enzymatic Activity of Phospholipase A2, a Putative Membrane-Associated Pressure Sensor, J. Am. Chem. Soc. 137 (2015) 12588-12599

[4] J. Möller, S. Grobelny, J. Schulze, S. Bieder, A. Steffen, M. Erlkamp, M. Paulus, M. Tolan, R. Winter, Reentrant Liquid-Liquid Phase Separation in Protein Solutions at Elevated Hydrostatic Pressures, Phys. Rev. Lett. 112 (2014) 028101

[5] M. A. Schroer, Y. Zhai, D. C. F. Wieland, C. J. Sahle, J. Nase, M. Paulus, M. Tolan, R. Winter, Exploring the Piezophilic Behavior of Natural Cosolvent Mixtures, Angew. Chem. Int. Ed. 50 (2011) 11413

[6] K. Julius, J. Weine, M. Berghaus, N. König, M. Gao, J. Latarius, M. Paulus, M. A. Schroer, M. Tolan, R. Winter, Water-Mediated Protein-Protein Interactions at High Pressures are Controlled by a Deep-Sea Osmolyte, Phys. Rev. Lett. ​​​​​​​121 (2018) 038101

[7] S. Patra, V. Schuabb, I. Kiesel, J. M. Knop, R. Oliva, R. Winter, Exploring the Effects of Cosolutes and Crowding on the Volumetric and Kinetic Profile of the Conformational Dynamics of a Poly dA Loop DNA Hairpin: A Single-molecule FRET Study, Nucl. Acids Res. 47 (2019) 981-996

[8] S. Cinar, H. Cinar, H. S. Chan, R. Winter, Pressure-Sensitive and Osmolyte-Modulated Liquid-Liquid Phase Separation of Eye-Lens Gamma-Crystallins, J. Am. Chem. Soc. 141 (2019) 7347-7354

[9] T. Q. Luong, N. Erwin, M. Neumann, A. Schmidt, C. Loos, V. Schmidt, M. Fändrich, R. Winter, Hydrostatic Pressure Increases the Catalytic Activity of Amyloid Fibril Enzymes. Angew. Chem. Int. Ed. 55 (2016) 12412



Philipps-Universität Marburg FB 15 Chemie, Hörsaal C Hans-Meerwein-Straße 4 35043 Marburg
Datum (Start der Veranstaltung)